2009年度森泰吉郎記念研究振興基金成果報告

2010年度 森泰吉郎記念研究振興基金 成果報告

左右相称動物で進化的に保存されたmiRNAとその起源

慶應義塾大学大学院政策メディア研究科

修士課程二年高根香織

Evolutionarily conserved microRNAs in bilaterian animals and their possible origin

Graduate School of Media and Governance, Keio university

Master’s Program 2nd-year

Kahori Takane

Abstract

microRNAs (miRNAs) are approximately 22 nucleotide non-coding RNAs that regulate gene expression at the posttranscriptional level. miRNAs are widely recognized as essential factors to understand living systems since its have been played important roles of cellular process such as cell development and differentiation. Recently, many miRNAs are found in not only animals but also plants and viruses. Currently, approximately 15,000 miRNA sequences have been registered in the database, and their number is increasing day by day. Although these miRNAs are mainly categorized into conserved miRNAs and lineage specific miRNAs, it is not obvious clear about miRNA evolution in bilaterian animals. We firstly reported that evolutionarily conserved miRNA/target-gene pairs were existed in differentiated cells such as miR-1–CNN3 expressed in muscle tissue and miR-124–GFPT2 expressed in Central nervous system. Since miRNA innovation is assumed to be involved with the morphological complexity observed throughout the metazoan evolution, analysis of the connection of the gain and loss of miRNAs and morphological features during the evolution of bilaterian animals is becoming an important issue. Therefore, we focused on Triops cancriformis (T. cancriformis) so-called Tadpole shrimp that still preserves ancestral morphology from approximately 200 million years ago. miRNA profiles of T. cancriformis were identified from each early developmental stage. We found novel highly abundant miRNA candidates that are expressed at specific stage(s). Furthermore, we have identified 13 miRNA candidates that are conserved only among T. cancriformis and vertebrates as well as 27 miRNA candidates that are conserved only among T. cancriformis and Caenorhabditis elegans indicating that the variation of evolutionarily conserved miRNAs among bilaterian animals are larger than previously expected.

研究業績

受賞歴
優秀修士論文, 2010年度

国際学会発表（筆頭著者のみ）
口頭発表
Takane K, Fujishima K, Watanabe K, Saito N, Sato A, Tomita M, Kanai A, Computational prediction and experimental validation of evolutionarily conserved microRNA target genes in bilaterian animals, 4thAYCOB (4th Asian Young Researchers Conference on Computational and Omics Biology), Singapore, 2010
*旅費及び滞在費全額補助

ポスター発表
Takane K, Fujishima K, Watanabe Y, Saito N, Sato A, Tomita M, Kanai A, Prediction and validation of evolutionary conserved microRNA target genes in bilateria, RNA conference 2010 (Fifteenth Annual Meeting of the RNA Society), Siattle USA, 2010

国内学会発表（筆頭著者のみ）
ポスター発表
高根香織, 平岡桐子, 広瀬由香, 佐藤朝子, 藤島皓介, 冨田勝, 金井昭夫, 左右相称動物で進化的に保存されたmiRNAとその起源の解析, 第32回日本分子生物学会年会第83回日本生化学会大会合同大会, 2010, 神戸

高根香織, 藤島皓介, 渡邊由香, 佐藤朝子, 斎藤信登, 冨田勝, 金井昭夫, 左右相称動物間で進化的に保存されたmiRNAとその標的遺伝子の予測と実験的検証, 第12回日本進化学会, 2010, 東京