Report of Taikichiro Mori Memorial Research Fund 2012
Research Project: |
Understanding the self-organisation of amino acid regulation in yeast |
Name: |
Kalesh Sasidharan, D3 |
Affiliation: |
Institute for Advanced Biosciences, Graduate school of media and governance, Keio University |
Introduction
The Taikichiro Mori Memorial Research Fund has helped me to improve my research by providing me the opportunity to purchase necessary equipments. I have purchased a computer that is suitable for the required data analyses. My research theme, progress and final goals are given below.
Research Project Abstract
When Saccharomyces cerevisiae are grown continuously respiration auto-synchronises resulting in stable oscillatory dynamics, where small molecules mediate communication [1]. Transcriptome-wide and metabolome-wide studies indicate that the oscillation functions to temporally separate catabolic and anabolic processes. Consistent with this, the production of amino acids has distinct phase relationships with the oscillation cycle [1]. In addition, oscillation is highly sensitive to amino acid and Rapamycin perturbations [1]. These data indicate a role for the master amino acid regulator Gcn4p in the regulation of oscillatory dynamics, where the Gcn4p is activated by non-aminoacylated tRNAs [2]. Therefore we explore the role of non-aminoacylated tRNAs in oscillatory regulation by constructing a regulatory model of amino acid regulation then testing this experimentally to provide a mechanistic understanding of amino acid feedback on gene regulation. We will also discuss the context of our work in the emergence of coherent behaviour in biochemical networks.
References
1. Douglas B Murray, Manfred Beckmann, and Hiroaki Kitano. Regulation of yeast oscillatory dynamics. Proc Natl Acad Sci U S A, 104(7):2241_2246, Feb 2007.
2. Alan G Hinnebusch. Translational regulation of gcn4 and the general amino acid control of yeast. Annu Rev Microbiol, 59:407_450, 2005.