2012年度森基金研究成果報告書
政策・メディア研究科 修士課程2年
先端生命科学(BI)
馬場藤貴
Isolation and identification of lactic acid bacteria from human
tear.
【要旨】
Commensal bacterial variation, function, and the exact percentage of
colonizedmicroflora on the human ocular surface remain
unclear. In our prior research, the types and features of ocular surface disease
and their relationship with microflora in atopic keratoconjunctivitis (AKC) have been evaluated using 16S
rRNA gene clone library analysis. We found that a kind
of lactic acid bacterial 16S rRNA gene was detected
from all of the human tear samples. However, the DNA sequences provided by the
16S rRNA gene clone library method may not come from
viable cells. To this end, we tried to culture ocular microflora of the human tear samples to prove the existence
of viable lactic acid bacteria on the ocular surface. We firstly isolated 96
bacterial strains from culture samples of human tear, and screened them by 16S
rRNA gene PCR method with lactic acid
bacteria-specific primers. As a result, 5 positive strains were selected;
however, further 16S rRNA gene full sequencing showed
that most of the strains were annotated as non-lactic acid bacteria, Staphylococcus epidermidis, which is well known as a commensal microbe
on the human skin. Therefore, we need more analysis to demonstrate the existence
of lactic acid bacteria on human ocular surface.
【詳細】
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