Report of Taikichiro Mori Memorial
Research Fund 2009
|
Research Project: |
Cellular
dynamics |
|
Project Name: |
Self-organisation
of amino acid regulation in yeast |
|
Name: |
Kalesh Sasidharan, D3 |
|
Affiliation: |
Institute
for Advanced Biosciences, Graduate school of media and governance, Keio
University |
Introduction
My research is based on experimental and computation
biology. The Mori grant has given me a good opportunity to improvise my research
by equipping the lab with necessary equipments. I have purchased several
computer peripherals, which are necessary for the data storage and data
acquisition. Apart of that, I have upgraded my computers and storage devices to
store and process huge amount of data files. My research theme, progress and
final goals are given below.
Research Project
Abstract
When Saccharomyces cerevisiae are grown continuously,
respiration autosynchronises resulting in stable oscillatory dynamics, where
small molecules mediate communication [1]. Transcriptome-wide and
metabolome-wide studies indicate that the oscillation functions to temporally
separate catabolic and anabolic processes. Consistent with this, the production
of amino acids have distinct phase relationships with the oscillation cycle
where amino acids are produced in conjunction with S-phase [1]. In addition,
oscillation is highly sensitive to amino acid and Rapamycin perturbation [1].
These data indicate a role for the master amino acid regulator Gcn4p in the
regulation of oscillatory dynamics, where the Gcn4p is activated by
non-aminoacylated tRNAs [2]. Therefore we explore the role of non-aminoacylated
tRNAs in oscillatory regulation by constructing a regulatory model of amino
acid regulation then testing this experimentally to provide a mechanistic
understanding of amino acid feedback on gene regulation. We will also discuss
the context of our work in the emergence of coherent behavior in biochemical
networks.
References
1. Douglas B Murray, Manfred Beckmann, and Hiroaki
Kitano. Regulation of yeast oscillatory dynamics. Proc Natl Acad Sci U S A,
104(7):2241_2246, Feb 2007.
2. Alan G Hinnebusch. Translational regulation of gcn4
and the general amino acid control of yeast. Annu Rev Microbiol, 59:407_450,
2005.